Using CRISPR to track embryo development

Kai Kupferschmidt, 26 May 2016, Science Magazine,

Tracing cells lineages from fertilization to adulthood has traditionally proven extremely challenging.  However McKenna et al. ( have now developed a technique named GESTALT (Genome Editing of Synthetic Target Arrays for Lineage Tracing) that uses CRISPR to create a unique barcode that can be followed through development.  The technique was developed in zebra fish by injecting the Cas9 enzyme and 10 unique gRNAs into single-celled embryos.  The Cas9/gRNA complexes can then cleave the targeted sequences through the first rounds of cell divisions over ~4 hours.  Each time a cleavage event occurs a unique InDel is created, thus allowing the lineage of organs and tissues to be determined from the sequence of the fully developed zebra fish.

Author: Advanced Analytical

Advanced Analytical Technologies, Inc. (AATI) simplifies complex genomics workflows to accelerate research and discovery in pharmaceuticals, life science, biofuels, biotechnology and healthcare.

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