Kaminski, R., et. al. Gene Therapy (2016) https://www.ncbi.nlm.nih.gov/pubmed/27194423
While CRISPR/Cas9 technology has been used to inactivate HIV in human tissue culture, inactivation in living animals is much more challenging. Using a transgenic mouse model containing HIV sequences Kaminski et al where able to use tail-vein injection with a Adeno-associate virus vector containing a shortened Cas9 and gRNAs to cleave HIV sequences in the spleen, liver, heart, lung, kidney, and circulating lymphocytes. This is the first report of in vivo inactivation of HIV using CRISPR/Cas9 technology.