Dong, D., et. al. Nature (2016) 532:522-526 http://www.ncbi.nlm.nih.gov/pubmed/27096363
Researchers elucidated the crystal structure of the Cas9 functional analog Cpf1 in complex with crRNA at a 2.38Å resolution. Cpf1 was isolated from Lachnospiraceae bacterium ND2006 and found to have a triangular shape with a positively charged central channel. Extensive intramolecular forces and Mg(H20)62+ stabilize the Cpf1-crRNA structure. The determination of this crystal structure will facilitate engineering of Cpf1 in order to improve its specificity and decrease off-target effects.