Researchers solve the crystal structure of Cpf1 in complex with CRISPR RNA

Dong, D., et. al. Nature (2016) 532:522-526

Researchers elucidated the crystal structure of the Cas9 functional analog Cpf1 in complex with crRNA at a 2.38Å resolution.  Cpf1 was isolated from Lachnospiraceae bacterium ND2006 and found to have a triangular  shape with a positively charged central channel.  Extensive intramolecular forces and Mg(H20)62+ stabilize  the Cpf1-crRNA structure.  The determination of this crystal structure will facilitate engineering of Cpf1 in order to improve its specificity and decrease off-target effects.

Author: Advanced Analytical

Advanced Analytical Technologies, Inc. (AATI) simplifies complex genomics workflows to accelerate research and discovery in pharmaceuticals, life science, biofuels, biotechnology and healthcare.

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