Synthetic RNA polymerase III promoters facilitate high efficiency CRISPR-Cas9 mediated genome editing in Yarrowia lipolytica

Schwartz, C.M. et al. ACS Synthetic Biology (2016) http://www.ncbi.nlm.nih.gov/pubmed/26714206

Yarrowia lipolytica is a versatile oleaginous yeast used to model lipid pathways and  produce polymers commercially. However, its use has been limited due to its reliance on selectable markers during genome engineering.  Researchers from the University of California-Riverside have used CRISPR/Cas9 technology to develop a new modular plasmid named pCRISPRyl that can induce homologous recombination with ~60% efficiency without the use of selectable markers.

Author: Advanced Analytical

Advanced Analytical Technologies, Inc. (AATI) simplifies complex genomics workflows to accelerate research and discovery in pharmaceuticals, life science, biofuels, biotechnology and healthcare.

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