Schwartz, C.M. et al. ACS Synthetic Biology (2016) http://www.ncbi.nlm.nih.gov/pubmed/26714206
Yarrowia lipolytica is a versatile oleaginous yeast used to model lipid pathways and produce polymers commercially. However, its use has been limited due to its reliance on selectable markers during genome engineering. Researchers from the University of California-Riverside have used CRISPR/Cas9 technology to develop a new modular plasmid named pCRISPRyl that can induce homologous recombination with ~60% efficiency without the use of selectable markers.