Screening for CRISPR Off-Target Sites with NGS Sequencing Chips

Phys.org, 29 June 2017 https://phys.org/news/2017-06-technique-enables-safer-gene-editing-therapy.html

CRISPR gene therapy has the potential to cure many genetic disorders, however the off-target activity of the CRISPR system must be understood before therapies can be deployed.  Scientists at the University of Texas at Austin have developed a technique to screen individuals for off-target sites using NGS chips and custom software called CHAMP, or Chip Hybridized Affinity Mapping Platform.  The authors used this technique to characterize the PAM requirements for the CRISPR molecule called Cascade.

Author: Advanced Analytical

Advanced Analytical Technologies, Inc. (AATI) simplifies complex genomics workflows to accelerate research and discovery in pharmaceuticals, life science, biofuels, biotechnology and healthcare.

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