David Cyranoski, Nature News, 8 August 2016, http://www.nature.com/news/replications-ridicule-and-a-recluse-the-controversy-over-ngago-gene-editing-intensifies-1.20387
While the CRISPR/Cas9 system has democratized and streamlined the gene editing process, scientists are always looking for the next big thing. In May of 2016 the gene editing community thought the next generation of gene editing tools may have been discovered with Gao et. al.’s Nature Biotechnology report on the DNA guided DNA targeting Argonaute protein isolated from Natronobacterium gregoryi (http://www.nature.com/nbt/journal/v34/n7/full/nbt.3547.html). However, few have been able to repeat the work. In an online survey conducted by the MRC Centre for Regenerative Medicine in the UK only 9 of 106 scientists reported success with the NgAgo gene editing system and Nature Biotechnology has begun an investigation into the report. The lack of reproducibility may simply be due to lack of details. Therefore, the authors released a detailed protocol to Addgene.