Rapid Detection and Quantification of CRISPR Induced Mutations

Luttgeharm, K.D. et. al, (2017) Biotechniques, 62:268-274. https://www.ncbi.nlm.nih.gov/pubmed/28625156

Detection of CRISPR mutations and the determination of mutation zygosity often slows gene editing workflows to a crawl.  In this Biotechniques report, Luttgeharm et. al. describe an optimized T7 Endonuclease I heteroduplex cleavage assay that detects a wide variety of mutations and predicts mutation zygosity for individual diploid cell lines and organisms.

Author: Advanced Analytical

Advanced Analytical Technologies, Inc. (AATI) simplifies complex genomics workflows to accelerate research and discovery in pharmaceuticals, life science, biofuels, biotechnology and healthcare.

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