Luttgeharm, K.D. et. al, (2017) Biotechniques, 62:268-274. https://www.ncbi.nlm.nih.gov/pubmed/28625156
Detection of CRISPR mutations and the determination of mutation zygosity often slows gene editing workflows to a crawl. In this Biotechniques report, Luttgeharm et. al. describe an optimized T7 Endonuclease I heteroduplex cleavage assay that detects a wide variety of mutations and predicts mutation zygosity for individual diploid cell lines and organisms.