Proximal Targeting With dCas Alters Chromatin Structure Allowing Genome Editing

Chen, Fuqiang, et. a. (2017) Nature Communications. 8:14958.

Many different Cas variants have been discovered, each with slightly different activities.  Francisella novicida Cas9 (FnCas9) is one such Cas variant. It is frequently inhibited at genomic loci, presumably due to chromatin formation.  By using dead SpCas9 to target proximally, FnCas9 could cleave at the target site, presumably due to chromatin rearrangements.

Author: Advanced Analytical

Advanced Analytical Technologies, Inc. (AATI) simplifies complex genomics workflows to accelerate research and discovery in pharmaceuticals, life science, biofuels, biotechnology and healthcare.

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