PAM sequence influences NHEJ repair mutation

Overbeek, M., et. al. (2016) Molecular Cell 63:633-646 http://www.ncbi.nlm.nih.gov/pubmed/27499295

NHEJ repairs have been considered a random process. However, a recent report indicates that NHEJ may be a non-random process.  The identification of the repair seems to be influenced by the PAM sequence and not the surrounding gDNA in human HEK293 and HCT116 cell lines with the repair being completed by either the classical NHEJ pathway or the microhomology mediated end-joining pathway.

Author: Advanced Analytical

Advanced Analytical Technologies, Inc. (AATI) simplifies complex genomics workflows to accelerate research and discovery in pharmaceuticals, life science, biofuels, biotechnology and healthcare.

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