Tycko, J., et. al. Molecular Cell (2016) 63:355-370. http://www.ncbi.nlm.nih.gov/pubmed/27494557
Since its discovery in 2012, CRISPR/Cas9 gene editing techniques and procedures have improved rapidly. Despite the rapid improvement, certain aspects of gene editing remain problematic. Off-target cleavage remains one of the most challenging problems especially in terms of gene editing’s therapeutic potential. Many different methodologies have been developed to screen for off-target effects, each with strengths and weaknesses. Tycko et al. review these techniques while also calling for standardized off-target detection methods.