Optimizing CRISPR/Cas9 target specificity

Tycko, J., et. al. Molecular Cell (2016) 63:355-370. http://www.ncbi.nlm.nih.gov/pubmed/27494557

Since its discovery in 2012, CRISPR/Cas9 gene editing techniques and procedures have improved rapidly. Despite the rapid improvement, certain aspects of gene editing remain problematic.  Off-target cleavage remains one of the most challenging problems especially in terms of gene editing’s therapeutic potential.  Many different methodologies have been developed to screen for off-target effects, each with strengths and weaknesses.  Tycko et al. review these techniques while also calling for standardized off-target detection methods.

Author: Advanced Analytical

Advanced Analytical Technologies, Inc. (AATI) simplifies complex genomics workflows to accelerate research and discovery in pharmaceuticals, life science, biofuels, biotechnology and healthcare.

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