Dhanjal JK., et. al. (2018) Genomics https://www.ncbi.nlm.nih.gov/pubmed/29605634
The ideal sgRNA will target a single loci in the genome and result in no off-target effects. Identifying such a sequence can involve significant amounts of trial and error. A new tool coined CRISPcut seeks to aid in this process by predicting sgRNAs for four different types of Cas9 nucleases and different PAM sequences in human cells. Additionally, experimental data restricts this tool to the open chromatin sequences and can predict sequences for paired nickases.