Huai, C. et. al. (2017) Nature Communications. 8:1375. https://www.ncbi.nlm.nih.gov/pubmed/29123204
Researchers have solved a 5.2 Å cryo-EM structure of Cas9 complexed with sgRNA and target DNA. The structure found that the HNH domain moves closer to the DNA than previously reported and suggests that this new structure resembles a DNA cleavage-activating structure of Cas9.