Natural CRISPR Diversity Could Expand the Gene Editing Toolbox

Karvelis, T. et. a. (2017) Current Opinions in Microbiology 37:88-94. https://www.ncbi.nlm.nih.gov/pubmed/28645099

While the CRISPR/Cas9 system is a significant improvement over previous gene editing technology, limitations remain. A significant constraint is the requirement for the PAM sequence.  By identifying Cas9 variants from additional species and in vitro evolution of identified variants, scientists will be able to diversify the number of sequences targeted.

Author: Advanced Analytical

Advanced Analytical Technologies, Inc. (AATI) simplifies complex genomics workflows to accelerate research and discovery in pharmaceuticals, life science, biofuels, biotechnology and healthcare.

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