Modeling CRISPR Hybridization Kinetics

Klein, M. et. al. (2018) Cell Reports 22:1413-1426. https://www.ncbi.nlm.nih.gov/pubmed/29425498

For CRISPR-based therapies to become viable, the off-target effects of the nucleases must be controlled.  To enhance the guide selection, this paper presents a kinetic model using four parameters that can mechanistically explain guide binding and off-target predictions.

Author: Advanced Analytical

Advanced Analytical Technologies, Inc. (AATI) simplifies complex genomics workflows to accelerate research and discovery in pharmaceuticals, life science, biofuels, biotechnology and healthcare.

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