Model Predicts Relationship Between Editing Speed and Off-Target Effects

David Ruth, 17 October 2017, PHYS.org, https://phys.org/news/2017-10-genome-efficient.html

Researchers at Rice University have used computational models to predict the speed at which the CRISPR/Cas system identifies and cleaves the targeted location.  The research, published in the Biophysical Journal (https://www.ncbi.nlm.nih.gov/pubmed/28978436), determined that by allowing CRISPR to cut at off-target sites the system could quickly find and cleave the targeted site.  By limiting the system’s ability to cleave off-target sites, the dissociation of Cas9 from DNA greatly decreased the speed at which on-target sites were identified.

Author: Advanced Analytical

Advanced Analytical Technologies, Inc. (AATI) simplifies complex genomics workflows to accelerate research and discovery in pharmaceuticals, life science, biofuels, biotechnology and healthcare.

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