Methods for computational sgRNA design

Chuai,G., et. al. Trends in Biotechnology (2016) http://www.cell.com/trends/biotechnology/abstract/S0167-7799(16)30090-7

Proper design of the sgRNA can make or break a CRISPR/Cas9 experiment; however there are many different guidelines for sgRNA design.  In silico analysis can use data gathered from previous experiments to predict guide efficiency and possible off-target effects, however many different sgRNA programs have been developed.  This review compares the available sgRNA analysis programs to aid the user in designing sgRNAs.

Author: Advanced Analytical

Advanced Analytical Technologies, Inc. (AATI) simplifies complex genomics workflows to accelerate research and discovery in pharmaceuticals, life science, biofuels, biotechnology and healthcare.

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