Guo, Q., et. al. (2018) Scientific Reports. 8:2080. https://www.ncbi.nlm.nih.gov/pubmed/29391533
Homology directed repair (HDR) has long been plagued by low efficiency, limiting its use in gene editing. Researchers working with induced pluripotent stem cells (iPSC) have found that by incubating cells at 32°C for 24-48 hours post-transfection, HDR efficiency can be increased by two- to ten-fold. This type of research could allow for more efficient use of CRISPR HDR.