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Viral diseases result in acute and chronic illnesses, often with limited treatment options. CRISPR/Cas9 technology could be used as to treat chronic viral diseases such as HIV/AIDS, Hepatitis B, and HPV. However, the research into these applications is still in its infancy. This review highlights the potential for CRISPR/Cas9 technology to be adapted for use as a eukaryotic antiviral therapy as well as the areas where more research is needed.