Phys.org, 7 February 2017, https://phys.org/news/2017-02-hurdles-crispr-gene-treatment.html
While CRISPR/Ca9 gene editing is much more efficient than previous gene editing technologies, delivery of the reagents to cells still poses a challenge to researchers. Now researchers at the University of Massachusetts Amherst have developed a system published in ACS Nano (https://www.ncbi.nlm.nih.gov/pubmed/28129503) that uses nanoparticles to deliver CRISPR/Cas9 across the cell membrane and nuclear envelope. To achieve this, the researchers modified Cas9 to interact with the nanoparticles. This modified Cas9, coined Cas9En, can be delivered into 90% of cells grown in culture with an editing efficiency of ~30%.