Functional genetic screens for enhancer elements in the human genome using CRISPR-Cas9.

Korkmaz, G. et al (2016) Nature Biotechnology. http://www.ncbi.nlm.nih.gov/pubmed/26751173

Enhancer elements are genomic sequences that regulate the transcription of distantly located genes by acting as binding sites for transcription factors within the chromatin loops.  Enhancer elements are thought to play a significant role in cancer development by altering gene expression. Study of enhancer elements has been limited due to challenges in modifying enhancer sequences.  CRISPR/Cas9 technology may have overcome this with Korkmaz et al presenting a procedure to rapidly modify enhancer elements to study their downstream effects.  The authors went on to characterize two enhancer elements in the p53 and ERα oncogenes.

Author: Advanced Analytical

Advanced Analytical Technologies, Inc. (AATI) simplifies complex genomics workflows to accelerate research and discovery in pharmaceuticals, life science, biofuels, biotechnology and healthcare.

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