Eliminating the CRISPR/Cas9 machinery for edited cells

Wang, Y., et. al. Scientific Reports (2016) 6:31145 http://www.ncbi.nlm.nih.gov/pubmed/27503169

Two of the challenges facing CRISPR/Cas9 gene editing are off-target effects and Cas9 toxicity due to prolonged Cas9/gRNA expression.  In order to prevent these adverse outcomes Wang et al. have developed a CRISPR/Cas9 system that will spontaneously eliminate itself co-transforming with a deletion construct that knocks out Cas9 expression during induced crossing over events.

Author: Advanced Analytical

Advanced Analytical Technologies, Inc. (AATI) simplifies complex genomics workflows to accelerate research and discovery in pharmaceuticals, life science, biofuels, biotechnology and healthcare.

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