Chen, S., et. al. (2016) Journal of Biological Chemistry, http://www.ncbi.nlm.nih.gov/pubmed/27151215
Traditional CRISPR/Cas9 mediated gene editing of mice has involved microinjection of the Cas9/gRNA complexes into zygotes. However, this approach is limited due to the expertise required for microinjection. Chen et al developed an electroporation based procedure to deliver the Cas9/gRNA complexes and target the Tyrosinase gene allowing for faster and more efficient gene editing. In 88% of edited embryos both alleles where modified with 42% repaired by HDR to knock-in precise changes. The authors hope that this methodology will allow for easier gene editing in mice and potentially other model mammalian systems.