Electroporation delivery of Cas9/gRNA Ribonucleoproteins into mouse zygotes

Chen, S., et. al. (2016) Journal of Biological Chemistry, http://www.ncbi.nlm.nih.gov/pubmed/27151215

Traditional CRISPR/Cas9 mediated gene editing of mice has involved microinjection of the Cas9/gRNA complexes into zygotes. However, this approach is limited due to the expertise required for microinjection.  Chen et al developed an electroporation based procedure to deliver the Cas9/gRNA complexes and target the Tyrosinase gene allowing for faster and more efficient gene editing.  In 88% of edited embryos both alleles where modified with 42% repaired by HDR to knock-in precise changes.  The authors hope that this methodology will allow for easier gene editing in mice and potentially other model mammalian systems.

Author: Advanced Analytical

Advanced Analytical Technologies, Inc. (AATI) simplifies complex genomics workflows to accelerate research and discovery in pharmaceuticals, life science, biofuels, biotechnology and healthcare.

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