Evers, B., et. al. Nature Biotechnology (2016) https://www.ncbi.nlm.nih.gov/pubmed/27111720
High-throughput knockout screens have become an important tool in identifying essential genes. Traditionally this process used shRNA to target genes with both CRISPR/Cas9 and CRISPRi currently emerging as alternatives. To determine which type of screen produces the most reliable results with fewer off-target effects and low noise Evers et al compared the three technologies in RT-112 cells. CRISPR/Cas9 was found to perform better than its inactive CRISPRi counterpart and traditional shRNA screens.