CRISPR/Cas9 outperforms both CRISPRi and traditional shRNA in a genome wide knockout screen to identify essential genes

Evers, B., et. al. Nature Biotechnology (2016)

High-throughput knockout screens have become an important tool in identifying essential genes.  Traditionally this process used shRNA to target genes with both CRISPR/Cas9 and CRISPRi currently emerging as alternatives.  To determine which type of screen produces the most reliable results with fewer off-target effects and low noise Evers et al compared the three technologies in RT-112 cells.  CRISPR/Cas9 was found to perform better than its inactive CRISPRi counterpart and traditional shRNA screens.

Author: Advanced Analytical

Advanced Analytical Technologies, Inc. (AATI) simplifies complex genomics workflows to accelerate research and discovery in pharmaceuticals, life science, biofuels, biotechnology and healthcare.

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