CRISPR/Cas9 has been adapted to aid in mapping of unknown genes

Catherine Offord, 5 May 2016,The Scientist,

Traditional gene mapping follows phenotypic traits through multiple generations and homologous recombination events.  Using the cells natural meiotic processes researchers are able to determine regions of the genome responsible for certain traits, however this process is limited since crossing over is a rare event and it is impossible to control where the event occurs.  Researchers have now used CRISPR/Cas9 to induce crossing over through HDR  to control where crossing over occurs.  Coupled with a GFP marker, the researchers were able to quickly identify a SNP in yeast responsible for manganese sensitivity.  Additional research is needed to adapt this technique to more complex systems such as human cell lines.

Author: Advanced Analytical

Advanced Analytical Technologies, Inc. (AATI) simplifies complex genomics workflows to accelerate research and discovery in pharmaceuticals, life science, biofuels, biotechnology and healthcare.

Leave a Reply