CRISPR Off-Target Affects May be Greater than Previously Assumed

EurekAlert, Columbia University Medical Center Press Release, 29 May 2017,

Since its discovery, the CRISPR/Cas9 system has been on an unprecedented pace towards clinical use, though a new Nature Letter may slow down this process.  In the letter, Unexpected mutations after CRISPR-Cas9 editing in vivo (, researchers completed whole genome sequencing of CRISPR edited mice and discovered 1,500 single nucleotide mutations and greater than 100 larger deletions and insertions with none of the off-target mutations predicted by computer algorithms.  While this study could change the way CRISPR therapies progress, further researcher is needed to confirm the results.

Author: Advanced Analytical

Advanced Analytical Technologies, Inc. (AATI) simplifies complex genomics workflows to accelerate research and discovery in pharmaceuticals, life science, biofuels, biotechnology and healthcare.

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