CRISPR Off Switch Could Decrease Off-Target Effects

GEN News Highlights, 29 December 2016, http://www.genengnews.com/gen-news-highlights/off-switch-found-for-common-version-of-the-crispr-cas9-system/81253625

A new Cell publication (http://www.cell.com/cell/abstract/S0092-8674(16)31683-X) has demonstrated a CRISPR off switch by studying self-targeting bacteria—bacteria where viruses defeated CRISPR and integrated into the bacterial genome.  In these self-targeting bacteria, viral DNA can be found at both the CRISPR locus and in additional locations within the genome.  The authors hypothesized that the virus had a method to deactivate the CRISPR mechanism, preventing the CRISPR system from cutting the integrated viral DNA, killing the bacterium itself.  Four CRISPR/Cas9 inhibitor proteins were discovered in the Listeria monocytogenes prophages, allowing for the potential use of these inhibitors in CRISPR gene editing experiments.

Author: Advanced Analytical

Advanced Analytical Technologies, Inc. (AATI) simplifies complex genomics workflows to accelerate research and discovery in pharmaceuticals, life science, biofuels, biotechnology and healthcare.

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