CRISPR gene editing of methanogenic archaea

Nayak, D.D. et. al. (2017) PNAS USA. https://www.ncbi.nlm.nih.gov/pubmed/28265068

CRISPR gene editing has become an essential tool for modification of eukaryotic systems, however little work has been done in Archaea.  Presented here is the development of a method for CRISPR gene editing of the methanogenic archaeon Methanosarina acetivorans. Using this method, the authors could generate both insertions and deletions by homology directed repair.  The development of these tools will hopefully help to create new opportunities in archaea studies

Author: Advanced Analytical

Advanced Analytical Technologies, Inc. (AATI) simplifies complex genomics workflows to accelerate research and discovery in pharmaceuticals, life science, biofuels, biotechnology and healthcare.

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