Abby Olena, The Scientist 3 April 2017 http://www.the-scientist.com/?articles.view/articleNo/49055/title/CRISPR-Screen-Detects-Functional-Gene-Regulation/
Determining the function of non-protein coding DNA has traditionally been a difficult task despite its regulatory role and implication in diseases such as cardiovascular, diabetes, and neurological disorders. By using dCas9 researchers could create a repressor form of dCas9 that facilitates methylation of histone H3 and an activator form of dCas9 which facilitates the acetylation of histone H3. These two forms of dCas9 allow for rapid screening of gene expression levels resulting from targeted changes in the epigenome. This research has been published in Nature Biotechnology (https://www.ncbi.nlm.nih.gov/pubmed/28369033).