Black et al., Cell Stem Cell (2016) http://www.cell.com/cell-stem-cell/pdfExtended/S1934-5909(16)30196-5
Conversion of somatic cells into induced pluripotent stem cells (IPCs) has enabled the creation of model systems that more closely mimic their natural counterparts. The creation of IPCs cell lines has traditionally involved the overexpression of transgenes which encode specific transcription factors. While effective, this process has been labor intensive and slow. In this publication Black et al. demonstrate that dCas9 can be used to activate endogenous transcription factors eliminating the need for transgenes. This procedure has the potential to greatly increase the speed at which IPCs cell lines are created.