Creation of Non-Mosaic CRISPR Edited Mice

Hashimoto, M., et al.  Developmental Biology (2016)

Gene editing of mice has traditionally been completed using electroporation or microinjection of the CRISPR machinery into zygotes. However, this can lead to a genetic mosaic where different cells contain different mutations.  The nature of these models hinders the downstream phenotype analysis.  To overcome this Hashimoto et. al. have developed a method to electroporate fertilized zygotes in vitro before the first round of DNA replication, allowing the creation of a non-mosaic edited mouse model.

Author: Advanced Analytical

Advanced Analytical Technologies, Inc. (AATI) simplifies complex genomics workflows to accelerate research and discovery in pharmaceuticals, life science, biofuels, biotechnology and healthcare.

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