Hashimoto, M., et al. Developmental Biology (2016) http://www.ncbi.nlm.nih.gov/pubmed/27474397
Gene editing of mice has traditionally been completed using electroporation or microinjection of the CRISPR machinery into zygotes. However, this can lead to a genetic mosaic where different cells contain different mutations. The nature of these models hinders the downstream phenotype analysis. To overcome this Hashimoto et. al. have developed a method to electroporate fertilized zygotes in vitro before the first round of DNA replication, allowing the creation of a non-mosaic edited mouse model.