Creation of Non-Mosaic CRISPR Edited Mice

Hashimoto, M., et al.  Developmental Biology (2016) http://www.ncbi.nlm.nih.gov/pubmed/27474397

Gene editing of mice has traditionally been completed using electroporation or microinjection of the CRISPR machinery into zygotes. However, this can lead to a genetic mosaic where different cells contain different mutations.  The nature of these models hinders the downstream phenotype analysis.  To overcome this Hashimoto et. al. have developed a method to electroporate fertilized zygotes in vitro before the first round of DNA replication, allowing the creation of a non-mosaic edited mouse model.

Author: Advanced Analytical

Advanced Analytical Technologies, Inc. (AATI) simplifies complex genomics workflows to accelerate research and discovery in pharmaceuticals, life science, biofuels, biotechnology and healthcare.

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