Cpf1 employs a DNA binding mechanism distinct from Cas9

Gao et al, Cell Research (2016) http://www.ncbi.nlm.nih.gov/pubmed/27444870

Cpf1 is a Cas9 analog with an unknown cleavage mechanism that uses a single 42 nt-44 nt crRNA recognizing a T-rich PAM . By studying the crystal structure of Cpf1 in complex crRNA and target DNA researchers were able to start characterizing the interaction between Cpf1 and target DNA sequences.  Binding to target DNA is initially disordered in the Cpf1-crRNA complex, upon target recognition an ordered conformation is established allow the PAM interacting domain to change from an open-to-closed conformation.  This mechanism is distinct from that found in Cas9.

Author: Advanced Analytical

Advanced Analytical Technologies, Inc. (AATI) simplifies complex genomics workflows to accelerate research and discovery in pharmaceuticals, life science, biofuels, biotechnology and healthcare.

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