Bridged Nucleic Acids Improve Cas9 Specificity

Cromwell, CR., et. al. (2018) Nature Communications. 9:1448.

Off-target effects resulting from CRISPR gene editing could hamper the systems adoption as a therapeutic technique.  In a recent Nature Communications publication, Cromwell et. al. demonstrate that use of next-generation bridged nucleic acids (BNA) and locked nucleic acids (LNA) at specifics spots in the crRNA can reduce off-target cleavage by multiple orders of magnitude.  The authors demonstrate that this is due to the modified nucleic acids slowing Cas9 kinetics.

Author: Advanced Analytical

Advanced Analytical Technologies, Inc. (AATI) simplifies complex genomics workflows to accelerate research and discovery in pharmaceuticals, life science, biofuels, biotechnology and healthcare.

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