Automated Screening for CRISPR Mutations

Steve Siembieda and Kyle Luttgeharm, GEN Tutorials, 1 Nov 2017. https://www.genengnews.com/gen-articles/rapid-screening-for-the-zygosity-of-crispr-mutations/6194

Screening for CRISPR edits remains a large bottleneck in the gene editing process.  This GEN tutorial walks through a novel method for simultaneously identifying clones containing edits, while predicting the zygosity of the mutation in diploid organisms.  The assay relies on statistical models that predict the frequency of heteroduplex formation versus duplex formation and a T7 Endonuclease I based cleavage assay coupled with the quantitative abilities of capillary electrophoresis.

Author: Advanced Analytical

Advanced Analytical Technologies, Inc. (AATI) simplifies complex genomics workflows to accelerate research and discovery in pharmaceuticals, life science, biofuels, biotechnology and healthcare.

Leave a Reply