Gao, F., et. al. (2016) Nature Biotechnology doi: 10.1038/nbt.3547 http://www.ncbi.nlm.nih.gov/pubmed/27136078
DNA editing by CRISPR/Cas9 has overtaken previous gene editing technologies as the most commonly used editing method. However despite its widespread use limitations remain. To overcome these limitations Gao et al. searched a different group of endonucleases called Argonautes. Argonautes are the main endonuclease involved in RNA-guided RNA silencing, however a subset of Argonautes have been identified that use single stranded DNA to target DNA in extremophile bacteria. Using BLAST Gao et al. identified an Argonaute protein from a non-extremophile that can cleave DNA both in vivo and in vitro. Further work is needed before it can be used in complex mammalian systems.