Swings, et. al. (2018) Nature Communications 9:2231. https://www.nature.com/articles/s41467-018-04651-5?_ga=2.91952154.1695293922.1528489497-266046807.1528489497
Precise editing with CRISPR through homology directed repair requires careful selection of gRNA sequences and template source. This can be a challenge as each new site requires the design of new gRNAs. To streamline this process, Swings et. al. describes a method using the Keio E. coli knockout collection and a gRNA that targets the flanking ends of the inserted kanamycin cassette. This collection and reagents can be used to alter each gene in the E. coli genome through homology directed repair with the researcher only having to design the homology template.