An Off the Shelf Method for Precise Gene Editing in E. coli

Swings, et. al. (2018) Nature Communications 9:2231. https://www.nature.com/articles/s41467-018-04651-5?_ga=2.91952154.1695293922.1528489497-266046807.1528489497

Precise editing with CRISPR through homology directed repair requires careful selection of gRNA sequences and template source.  This can be a challenge as each new site requires the design of new gRNAs.  To streamline this process, Swings et. al. describes a method using the Keio E. coli knockout collection and a gRNA that targets the flanking ends of the inserted kanamycin cassette.  This collection and reagents can be used to alter each gene in the E. coli genome through homology directed repair with the researcher only having to design the homology template.

Author: Advanced Analytical

Advanced Analytical Technologies, Inc. (AATI) simplifies complex genomics workflows to accelerate research and discovery in pharmaceuticals, life science, biofuels, biotechnology and healthcare.

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