An Agrobacterium based CRISPR gene editing method for Maize

Char, S. N., et. al. Plant Biotechnology Journal, (2016) http://www.ncbi.nlm.nih.gov/pubmed/27510362

While the CRISPR/Cas9 gene editing system has been shown to work in a variety of cell types, including plants and animals, differences in plant biology require methods optimized for individual species.  Using a binary-vector, Agrobacterium delivery system Char et al. where able to achieve mutation frequencies greater than 70%.  To decrease the cost of maize transformation Char et al. demonstrate that it is also possible to mix two Agrobacterium strains to create transgenic plants independently mutated in each target.

Author: Advanced Analytical

Advanced Analytical Technologies, Inc. (AATI) simplifies complex genomics workflows to accelerate research and discovery in pharmaceuticals, life science, biofuels, biotechnology and healthcare.

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