Adaptation of CRISPR for use in neural tissue

25Emma Yasinki, GEN, May 25, 2016

CRISPR gene editing has now been used extensively to knock-in novel DNA sequences. However, as this mechanism relies in part on the cells native homologous recombination machinery, cells that don’t divide rapidly, such as neurons, have been difficult to modify.  Researchers have now developed a new technique coined SLENDR that uses CRISPR/Cas9 and in utero electroporation allowing transformation of prenatal models when the neural cells are still dividing thus allowing homology directed repair of double stranded breaks.

Author: Advanced Analytical

Advanced Analytical Technologies, Inc. (AATI) simplifies complex genomics workflows to accelerate research and discovery in pharmaceuticals, life science, biofuels, biotechnology and healthcare.

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